Over the last two decades Biotechnology has evolved as an important scientific discipline with intimate overlapping interactions with several other disciplines of Biology. In order to have clarity of understanding in this field, at least optimal knowledge in different related/ allied areas of biology is essential. Often it is being felt that a B Sc. Biotechnology student while pursuing his/her masters in Biotechnology often stumbles at several terms and terminology from pure Botany/ Zoology/ Microbiology. Considering this aspect, as many as possible, have been incorporated in this glossary, from these areas for their ready reference. Hence, we the authors engaged in teaching Biotechnology and research in different fields within the broad domain of biology have made an attempt for the first time, to carefully and comprehensively compile a glossary referring a broad spectrum of sources of texts, journals and websites. This glossary, which at the outset will definitely be helpful to the students of Biotechnology at the UG, PG, M. Phil. and the researchers pursuing Ph. D course work as well as catering to certain needs of the NET and GATE aspirants, and also be useful to the teachers